Puerarin modulates gut-liver-ovarian axis to improve reproductive performance in aged breeder hens (microbiomejournal.biomedcentral.com)

• 200 mg/kg puerarin improved egg quality and laying rate in aged hens
• Multi-omics revealed carbon flux redirection and increased vitellogenin synthesis
• Gut microbiota normalized (Lactobacillus, Bacteroides) and PPAR pathway regulated

"Dietary supplementation of 200 mg/kg puerarin, a natural isoflavone from Pueraria lobata, improved egg quality, laying rate, and feed conversion efficiency in aged hens. Multi-omics analysis (16S rRNA, transcriptomics, liver metabolomics) revealed that puerarin redirected hepatic carbon flux toward vitellogenin synthesis, normalized gut microbiota (Lactobacillus and Bacteroides), and regulated PPAR signaling. Data deposited in NCBI SRA and NGDC OMIX."